Document Details

Document Type : Thesis 
Document Title :
BIOINFORMATICS ANALYSIS OF PATHOGENIC MUTANT FORMS OF HUMAN IL10RA PROTEIN
تحليل معلوماتي حيوي لأشكال الطفرات الممرضة لبروتين IL10RA البشري
 
Subject : Faculty of Sciences > Biochemistry department 
Document Language : Arabic 
Abstract : Objective: The computational investigation of genetic mutation consequences on structural level of proteins is recently found to be an effective alternate to traditional in-vivo and in-vitro approaches. Hence, we have performed the in-silico analysis of clinically potential missense and un-translated region mutations of human IL10RA protein. Methodology: A combination of empirical rule and support vector machine based in-silico algorithms were employed in this study to identify the pathogenic non-synonymous genetic mutations. Additionally, molecular modeling and secondary structure analysis was also performed to confirm their impact on the stability and secondary properties of IL10RA protein. Results: Besides, the mutations corresponding to p.Y57C, p.T84I, p.Y91C, p.R101W, p.R117C, p.R117H and p.G141R in exonic region; c.*1537T>C, a regulatory region variant was also found to potentially influence the structural and functional deviations of IL10RA activity. Moreover, the molecular docking analysis of IL10RA with combinational substrates has revealed that peptide inhibitors compared to small non-peptide inhibitor molecules possess good inhibitory activity towards mutant IL10RA Conclusion: Our findings are expected to help in narrowing down the number of IL10RA genetic variants to be screened for disease association studies and also to select better competitive inhibitors for IL10RA related diseases. 
Supervisor : Dr. Fahad Ahmed M. Al-Abbasi 
Thesis Type : Master Thesis 
Publishing Year : 1436 AH
2014 AD 
Co-Supervisor : dr. 
Added Date : Monday, February 2, 2015 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
كليم الدين محمد ...., KALEEMUDDIN MOHAMMEDResearcherMaster 

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