Document Details

Document Type : Thesis 
Document Title :
STUDY OF SENESCENCE MARKER PROTEIN-30 IN RELATION TO VITAMIN D DEFICIENCY AND OBESITY IN ANIMAL MODEL
دراسة دلالة الشيخوخة الخلوية- 30 وعلاقته بنقص فيتامين د والسمنة في النموذج الحيواني
 
Subject : Faculty of medicine 
Document Language : Arabic 
Abstract : Background and aim: Obesity is a pandemic disease and the international data predicted that by 2030 the majority of adults will be either obese or overweight. Vitamin D deficiency is also a significant health problem worldwide and has been implicated in a wide variety of disease states. An association between vitamin D deficiency and obesity is well known, but the mechanisms are not totally clear. Cellular senescence of adipose tissue is considered as a major mechanism that contributes to development and progression of obesity and has been recently linked to vitamin D. The current study aimed to study the possible link between obesity and cellular senescence as well as the possible role of vitamin D deficiency in the pathogenesis of obesity-related to cellular senescence. Materials and Methods: A total number of 47 male albino rats were enrolled in this case-control study. 27 albino rats were fed high-fat diet (high fat diet group) and 20 albino rats were fed an ordinary balanced diet (control group). All rats were subjected to anthropometric measurements including body weight, length and body mass index (BMI). Serum glucose, triglycerides, total cholesterol, HDL-cholesterol, LDLcholesterol were estimated colorimetrically. While, serum insulin, vitamin D and Senescence Marker Protein 30 (SMP30) were estimated by using enzyme-linked immunosorbent assay (ELISA). Results: There was statistically significant increase in BMI in obese rats (0.71 ± 0.03 g\cm 2 ) compared to controls (0.62 ± 0.03 g\cm 2 ), (P < .001). SMP30 levels were significantly lower in obese rats (70.00 ± 41.32 pg\ml) compared to controls (176.35 ± 50.62 pg\ml), (P < .001). In addition, vitamin D levels were significantly lower in obese rats (14.99 ± 2.77 nmol\l) compared to controls (40.27 ± 6.29 nmol\l), (P < .001). Moreover, SMP30 and vitamin D showed significant negative correlation with BMI (P = .005), (p < .001); respectively in both groups. Furthermore, vitamin D showed significant positive correlation with SMP30 (P = .002) in both groups. Conclusion: It could be concluded that vitamin D deficiency and enhanced cellular senescence are common pathological features of obesity. Hypovitaminosis D and increased cellular senescence can be a cause or effect of obesity which need further investigations. 
Supervisor : Dr. Fayza Fawaz Alfayez 
Thesis Type : Master Thesis 
Publishing Year : 1441 AH
2020 AD 
Added Date : Monday, January 20, 2020 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
تغريد محمد خواجيKhawaji, Taghreed MohammedResearcherMaster 

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