Document Details
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Document Type |
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Thesis |
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Document Title |
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Studying the Effect of Zingerone on Diabetic Cardiovascular Complications in Rat دراسة تأثير الزنجرون على مضاعفات القلب الوعائية المحدثة بالسكري في الجرذان |
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Subject |
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Faculty of Sciences |
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Document Language |
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Arabic |
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Abstract |
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The present study was planned to investigate the effects of zingerone administration (Z) on cardiac complication in streptozotocin induced rats)D). Material and Methods: Male Wistar rats, 6 weeks age, weighing 180 g (n=8) were classified into four groups; control, diabetic rats, diabetic treated with (Z, 20mg/kg/day), and control treated with (Z, 20mg/kg/day). Diabetes was induced experimentally in rats by STZ injection (50 mg/kg i.p. injection. After an additional 7 weeks, blood and tissue were collected for biochemical analysis and 8-isoprostenol was measured in urine. Cardiac hemodynamics and ECG were assessed. Expression of cardiac and coronary artery (angiotensin receptor 1 (AT1) and TGFβ1) were assessed by immunofluorescence. Histopathological examination of heart and aorta were also carried out. Results: results reported that the STZ-induced diabetic group presented a hugely significant increase (p<0.05) in hyperglycemia, accompanied with weight loss when compared with control group. Diabetes produced a significant increase (p<0.05) in QT, QTc JT intervals, T peak Tend interval compared to control and changes were significantly modified by treatment with Zingerone. HR was significantly declined in STZ induced diabetic rats compared to control while treatment with z showed no effect. The increase in Tau was confirming the damaging effects of diabetes on cardiac structure and function. Z did not show any effect of cardiac contractility parameters (Max dP/dt, Min dP/dt, and Tau ). Moreover, diabetic model treated with (z) showed a significant increase (p<0.05) in serum adiponectin and recorded non-significant reduction of cardiac (TGFβ1&NF-κB) levels compared to D. Diabetic rats had a significant increase (p<0.05) in urinary 8-isoprostane levels and these increased levels were significantly changed by treatment with Z. zingerone successfully prevented oxidative stress, which was evidenced by an increase in glutathione reduced and, catalase levels. Moreover, zingerone suppressed the overexpression of AT1 receptor and TGFβ1 in the heart tissue. Histopathological investigation of diabetic heart and aorta represented the presence of some changes, the majority of sections tend to be normal, and this improvement in the diabetic treated cardiac sections may explain the antioxidant effect of the zingerone under study. In conclusion, the present study demonstrates that oral administration of the zingerone alleviates the delayed cardiac repolarization associated with diabetes. The protective zingerone effect seems to be mediated by its antioxidant and anti-inflammatory effect.
Key words: diabetes, zingerone, histopathology, delayed cardiac repolarization and hyperglycemia. |
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Supervisor |
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Dr. Mohammad Ibrahim Mujallid |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1438 AH
2017 AD |
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Co-Supervisor |
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Dr. Hany Mohammad El-Bassossy |
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Added Date |
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Tuesday, February 28, 2017 |
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Researchers
| وفاء سفر الذبياني | Althubiani, Wafa Safar | Researcher | Master | |
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