Document Details

Document Type : Thesis 
Document Title :
EVALUATION OF SERUM LEVEL OF IL-6 AND ITS SOLUBLE RECEPTOR BEFORE AND AFTER TREATMENT WITH MESENCHYMAL STEM CELLS IN THE RAT MODEL OF ARTHRITIS.
تقييم معدلات الانترليوكين 6 و مستقبلاتها في مصل الدم قبل وبعد العلاج باالخلاياالميسينكايمل الجذعية لدي فئران تجارب مصابين بداء التهاب المفاصل
 
Subject : Faculty of medicine 
Document Language : Arabic 
Abstract : Osteoarthritis (OA) is an inflammation of joint. Several proinflammatory cytokines are involved such as interleukin-6 (IL-6). IL-6 is a multifunctional proinflammatory cytokines. Its action responses are regulated by the recognition of a soluble interleukin 6 receptor (Sil-6R) to IL-6, which forms a ligand–receptor complex, these ligand are able to stimulate different cellular responses including proliferation of inflammatory cell, differentiation and activation of inflammatory processes. Mesenchymal stem cells (MSCs) are strong regulators of inflammation through the suppression of adaptive and innate immune systems. They have a role in the treatment of some chronic illnesses in general, where conventional therapy fails. Their role in alleviation of osteoarthritis needs to be investigated. The objectives of this study is to estimate reparative actions of MSCs on the process of knee OA using (IL-6 & Sil-6R) as indicator for OA inflammation Method: A total of 24 Healthy male rats were used for the experiment. Controlled animal study is done. They were divided into three groups (control, untreated OA groups and treated OA groups). Knee OA were induction by using chemically of Sodium Monoidoacetate (MIA). MSCs were isolated from legs bon of healthy albino rats. The level of IL-6 and Sil-6R were determined by sandwich ELISA method. Knee histological examination stained by hematoxylin and eosin (H&E) which examined by microscope. Result: The results shows that IL-6 parameters appeared to increasingly improved after MSCs injection, where the level of Sil-6 R was Continue to increased even after the injection of MSCs. The histological examination showed disrupt and irregular articular cartilage with reduce number of chondrocyte in OA groups. Where MSCs injection caused increase proliferation of chondrocyte, normalize articular surface and regeneration of articular cartilage. Conclusion: Intra-articular injection of culture-expanded MSCs in OA knees is an effective method for cartilage repair in rats, which the level of pro-inflammatory (IL-6) decrease after MSCs injection. This makes it a very promising tool for the treatment of patients with OA. 
Supervisor : Prof. AbduWhab Noor wali 
Thesis Type : Master Thesis 
Publishing Year : 1439 AH
2018 AD 
Added Date : Monday, June 4, 2018 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
تغريد مشعل باشهBashah, Taghreed MeshalResearcherMaster 

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